A fluorometric rate assay of peroxidase using the homovanillic acid-o-dianisidine-hydrogen peroxide system.
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Hydrogen peroxide assay by using enhanced chemiluminescence of the luminol-H202--horseradish peroxidase system: Comparative studies
p-Iodophenol, p-coumaric acid, phenol and aniline are enhancers of the luminol-H202-horseradish peroxidase chemiluminescence. These enhanced and unenhanced chemiluminescences were used to assay low concentrations of hydrogen peroxide. The curves of cherniluminescence intensity maxima against hydrogen peroxide concentration were fitted to third order polynomial regressions, which permit a very g...
متن کاملA Modified Fluorimetric Method for Determination of Hydrogen Peroxide Using Homovanillic Acid Oxidation Principle
Hydrogen peroxide (H2O2) level in biological samples is used as an important index in various studies. Quantification of H2O2 level in tissue fractions in presence of H2O2 metabolizing enzymes may always provide an incorrect result. A modification is proposed for the spectrofluorimetric determination of H2O2 in homovanillic acid (HVA) oxidation method. The modification was included to precipita...
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Featured Article: Fossati P, Prencipe L, Berti G. Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clin Chem 1980;26:227–31. Our 1980 report in Clinical Chemistry described an improved chromogenic detection system that, coupled with the enzyme oxidation of uric acid, led to a direct method for assaying ...
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A micromachined flow cell (overall size; 25 x 25 x 1 mm3) was designed for the fast determination of hydrogen peroxide, based on a luminol-H2O2 chemiluminescence reaction catalyzed by immobilized peroxidase (POD). The flow cell consisted of a sandwich of anisotropically etched silicon and glass chips and contained a spiral channel (20 turns, 50 cm long, 150 microm wide, 20 microm depth, channel...
متن کاملModeling the reduction of hydrogen peroxide by glutathione peroxidase mimics.
Theoretical calculations have been performed on three model reactions representing the reduction of hydrogen peroxide by ebselen, ebselen selenol, and ebselen diselenide. The reaction surfaces have been investigated at the B3PW91/6-311G(2df,p) level, and single-point energies were calculated using the 6-311++G(3df,3pd) basis set. Solvent effects were included implicitly with the conductor-like ...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1983
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.31.3579